Rapid Test for Organophosphate and Carbamate Pesticides

1 Scope of application: This method is suitable for organophosphorus and carbamate pesticide detection in vegetables, fruits, appropriate food, water and toxic residues. It is taken from fast national standard detection methods GB/T5009.199-2003.
2 Detection principles

3 test specimens
3.1 Rapid detection of pesticides card
3.2 Ethyl acetate
3.3 pH7.5 phosphate extraction （buffer） solution: 15.0g disodium hydrogen phosphate were taken [Na2HPO4 • 12H2O] and 1.59g of potassium dihydrogen phosphate [KH2 PO4], with 500mL distilled water.
3.4 Balance

3.5 Rapid Test for pesticides designed "pesticide residues Tachometer" and ultrasonic extractor.
4 Fast tests of pesticide residues in vegetables and fruits. Judgment of the results and Notes.
4.1 Determination by the surface （coarse sieve method）: vegetables, wipe the surface soil, drop 2 or 3 drops of extract in vegetable surface, with the other pieces of vegetables gently in the drip Department of friction. Take a quick test card, the vegetables on the liquid drops on the white pills. 10min placed pre-reaction, the Rapid Test Pack （red and white tablets pills superimposed）, the Yong Shounie 3min, open the card compared with the control experiment; if the white tablets change into light blue or does not change color, then the results are positive. If the white tablet changes into blue or the same as the control card, then the result is negative. Conditionally, it will slip into "pesticide residues Tachometer" automatic thermostat, timing detection.
4.2 Determination by the whole: select representative samples of vegetables, wipe the surface of the soil, cut into 1cm square pieces around, take into Lid 5g bottle, add 10mL extract （extract of the sample and the ratio is 1 + 2）, shake 50 times （conditional, can be extracted in the extractor bottle into the ultrasonic vibration for 30 seconds）, stand more than 2min. Take a quick test card drops on the white pills on 2 to 3 drops of extract, pre-placement 10min reaction, the Rapid Test Pack （red and white tablets pills superimposed）, the Yong Shounie 3min, open and empty control experiments card comparison, if the white tablets change into light blue or does not change color, then the results are positive. If the white tablet changes into blue or the same as the control card, then the result is negative. Conditionally, it will slip into "pesticide residues Tachometer" automatic thermostat, timing observation.
4.3 Note:
4.3.1 Using pesticide residue determination methods at home and abroad （disk diffusion method and spectrophotometry） are in the same test principle, determination of interfering substances are basically the same. Onion, garlic, radish, celery, parsley, water bamboo, mushrooms and tomato juice contains the enzyme affect plant secondary substances prone to false positives. When dealing with such samples （including those with high chlorophyll vegetables）, do not cut in too broken. Determination of tomato, the eggplant can extract on the City Department soak 2min, get soaking solution was measured. Determination of chives or garlic, it can put the whole container, whole root, or by adding extract measured after the shaking extraction.
4.3.2 Detection of pesticides in drinking water can be directly added to take 2 to 3 drops of speed measurement card to operate. Tea on the detection of pesticides in tea, can be measured this way, processed tea, the light of "the corresponding residues in food and pesticide poisoning detection."
4.3.3 Detection Rapid Test samples placed in pre-reaction time should be placed with the control card as the same time. Red pills and white composite reaction time control pills in 3min, open observation time should be within 1min prevail.
4.3.4 control card does not change color because, first, tablets may increase the surface extract less, pre-reaction of the tablet surface moist enough; second extract （buffer） of pH may be a problem （when available pure water for comparison to confirm）; Third, the operating environment in the air may contain pesticide residues.
4.3.5 In determining the sample of organic phosphorus or carbamate pesticides positive results, should be a repetition of the result of multiple detections, if necessary, the samples sent to the laboratory when using gas Se Pu Huo mass spectrometer instrument to further determine which Nongyao, the exact content.
5 The corresponding residues in food and pesticide poisoning, and the results determine and notes:
5.1 Sample handling and determination: to take the sample amount in the container, add 2 times the amount of ethyl acetate, after sufficient shaking standing, take mix with the pan in water bath on evaporation to dryness in ethyl acetate, to take 1mL phosphate extract after evaporation to dryness the residue dissolved, take 2 to 3 drops of solution residue on the white pills on a quick test card, placing 10min pre-reaction, the Rapid Test Pack （red and white tablets pills superimposed）, hand pinching 3min, open the speed test card, and also control the operation speed test card （white tablet on an increase of only extract the Rapid Test） compared to white pills do not change color as the strong positive results, a slight light blue for the weak positive The results, sky blue or the same with the control card for the negative results.
5.2 Notes:
5.2.1 In determining the sample for the organophosphate or carbamate pesticides positive results, testing should be repeated many times the result of the poisoning, residues, essential to the sample sent to the laboratory using gas chromatography or mass spectrometry to further determine which pesticides, the exact content.
5.2.2 Other Considerations and vegetables and fruits in the determination of pesticide residues in the same note.
6 Pesticide quality control of Rapid Test:
6.1 How and attention issues, only add pH7.5 phosphate solution or pure water extraction of pesticides strain Rapid Test for the blue; 0.3mg/kg of dichlorvos, or trichlorfon solution, enables fast testing of pesticides card positive.
7. Product storage and Validity: Closed Light Rapid Test is valid for 1 year storage at room temperature. The storage can be extended by cold storage.